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ProductsBack/TPMT Mouse Monoclonal Antibody [Clone ID: OTI2A2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TPMT (Cat# RC203309, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT(Cat# TA500966). Positive lysates [LY400131] (100ug) and [LC400131] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TPMT (Cat# RC203309, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT(Cat# TA500966). Positive lysates [LY400131] (100ug) and [LC400131] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TPMT (Cat# RC203309, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT(Cat# TA500966). Positive lysates [LY400131] (100ug) and [LC400131] (20ug) can be purchased separately from OriGene.

TPMT Mouse Monoclonal Antibody [Clone ID: OTI2A2]

CF500966
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTPMT
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    TPMT Mouse Monoclonal Antibody [Clone ID: OTI2A2]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2A2
  • Gene ID7172
  • Target name
    TPMT
  • Target description
    thiopurine S-methyltransferase
  • Target synonyms
    TPMTD, thiopurine S-methyltransferase, S-adenosyl-L-methionine:thiopurine S-methyltransferase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP51580
  • Protein Name
    Thiopurine S-methyltransferase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) TPMT mouse monoclonal antibody, clone OTI2A2 (formerly 2A2)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of TPMT using anti-TPMT antibody (A00671-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SGC-7901 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPMT antigen affinity purified polyclonal antibody (Catalog # A00671-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPMT at approximately 28KD. The expected band size for TPMT is at 28KD.
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