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ProductsBack/TPSAB1 / Mast Cell Tryptase Antibody

TPSAB1 / Mast Cell Tryptase Antibody

LS-C835157
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetTPSAB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TPSAB1 / Mast Cell Tryptase Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:100 - 1:300)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    0.6 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7177
  • Target name
    TPSAB1
  • Target description
    tryptase alpha/beta 1
  • Target synonyms
    TPS1, TPS2, TPSB1, TPSB2, Tryptase-2, tryptase alpha/beta-1, Tryptase II, Tryptase beta-2, epididymis secretory sperm binding protein, mast cell alpha II tryptase, mast cell beta I tryptase, mast cell tryptase, tryptase alpha II, tryptase alpha-1, tryptase beta I, tryptase beta-1, tryptase-1, tryptase-I, tryptase-III
  • Host
    Mouse
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mast Cell Tryptase using anti-Mast Cell Tryptase antibody (PB10016). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mast Cell Tryptase antigen affinity purified polyclonal antibody (Catalog # PB10016) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mast Cell Tryptase at approximately 30 kDa. The expected band size for Mast Cell Tryptase is at 31 kDa.
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