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Gel: 6%SDS-PAGE Lysate: 40 microg Lane 1-2: K562 cells A431 cells Primary antibody: TA365092 (TPX2 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds
Gel: 6%SDS-PAGE Lysate: 40 microg Lane 1-2: K562 cells A431 cells Primary antibody: TA365092 (TPX2 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds
Gel: 6%SDS-PAGE Lysate: 40 microg Lane 1-2: K562 cells A431 cells Primary antibody: TA365092 (TPX2 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

TPX2 Rabbit Polyclonal Antibody

TA365092
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetTPX2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    TPX2 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID22974
  • Target name
    TPX2
  • Target description
    TPX2 microtubule nucleation factor
  • Target synonyms
    C20orf1; C20orf2; differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; DIL2; DIL-2; FLS353; GD:C20orf1; HCA519; HCTP4; hepatocellular carcinoma-associated antigen 519; hepatocellular carcinoma-associated antigen 90; p100; preferentially expressed in colorectal cancer; protein fls353; REPP86; restricted expression proliferation associated protein 100; targeting protein for Xklp2; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULW0
  • Protein Name
    Targeting protein for Xklp2
  • Scientific Description
    TPX2 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TPX2 using anti-TPX2 antibody (A01610-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human SGC-7901 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPX2 antigen affinity purified polyclonal antibody (Catalog # A01610-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPX2 at approximately 86KD, 100KD. The expected band size for TPX2 is at 86KD.
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