TRAcP Antibody / TRAP / ACP5

Biorbyt

TRAcP Antibody / TRAP / ACP5

16

Optimal dilution of the recombinant TRAcP antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT IHC-P

Research Use Only

Monoclonal

rACP5/1070

Unconjugated

ACP5

acid phosphatase 5, tartrate resistant

HPAP, TRACP5a, TRACP5b, TRAP, TRAcP, TrATPase, tartrate-resistant acid phosphatase type 5, human purple acid phosphatase, tartrate-resistant acid ATPase, tartrate-resistant acid phosphatase 5a, tartrate-resistant acid phosphatase 5b

Mouse

IgG1

Tartrate-resistant acid phosphatase type 5

It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This MAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gauchers Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.

Human, Mouse, Rat

-20°C,2°C to 8°C

12352203