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ProductsBack/TRAIL antibody [6G24]

TRAIL antibody [6G24]

GTX53436
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetTnfsf10
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRAIL antibody [6G24]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6G24
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID22035
  • Target name
    Tnfsf10
  • Target description
    tumor necrosis factor (ligand) superfamily, member 10
  • Target synonyms
    A330042I21Rik, APO-2L, Ly81, TL2, Tnlg6a, Trail, tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, TRAIL/APO2L, tumor necrosis factor ligand 6a
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDP50592
  • Protein Name
    Tumor necrosis factor ligand superfamily member 10
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAIL using anti-TRAIL antibody (A00466). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPA1-6 whole Cell lysates, Lane 2: NIH3T3 whole Cell lysates, Lane 3: PC12 whole Cell lysates, Lane 4: NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAIL antigen affinity purified polyclonal antibody (Catalog # A00466) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAIL at approximately 20KD. The expected band size for TRAIL is at 35KD.
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