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ProductsBack/Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Polyclonal Antibody

Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Polyclonal Antibody

CAU28485
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetTnfsf10
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Overview

  • Supplier
    Biomatik
  • Product Name
    Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22035
  • Target name
    Tnfsf10
  • Target description
    tumor necrosis factor (ligand) superfamily, member 10
  • Target synonyms
    A330042I21Rik, APO-2L, Ly81, TL2, Tnlg6a, Trail, tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, TRAIL/APO2L, tumor necrosis factor ligand 6a
  • Host
    Rabbit
  • Protein IDP50592
  • Protein Name
    Tumor necrosis factor ligand superfamily member 10
  • Scientific Description
    The Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAIL using anti-TRAIL antibody (A00466). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPA1-6 whole Cell lysates, Lane 2: NIH3T3 whole Cell lysates, Lane 3: PC12 whole Cell lysates, Lane 4: NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAIL antigen affinity purified polyclonal antibody (Catalog # A00466) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAIL at approximately 20KD. The expected band size for TRAIL is at 35KD.
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