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ProductsBack/Transient Receptor Potential Cation Channel Subfamily V, Member 6 (TRPV6) Polyclonal Antibody

Transient Receptor Potential Cation Channel Subfamily V, Member 6 (TRPV6) Polyclonal Antibody

CAU22383
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetTRPV6
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Overview

  • Supplier
    Biomatik
  • Product Name
    Transient Receptor Potential Cation Channel Subfamily V, Member 6 (TRPV6) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.29 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55503
  • Target name
    TRPV6
  • Target description
    transient receptor potential cation channel subfamily V member 6
  • Target synonyms
    ABP/ZF, CAT1, CATL, ECAC2, HRPTTN, HSA277909, LP6728, ZFAB, transient receptor potential cation channel subfamily V member 6, Alu-binding protein with zinc finger domain, calcium transport protein 1, calcium transporter-like protein, epithelial apical membrane calcium transporter/channel CaT1, epithelial calcium channel 2
  • Host
    Rabbit
  • Protein IDQ9H1D0
  • Protein Name
    Transient receptor potential cation channel subfamily V member 6
  • Scientific Description
    The Transient Receptor Potential Cation Channel Subfamily V, Member 6 (TRPV6) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPV6 using anti-TRPV6 antibody (A02139-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HGC-27 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV6 antigen affinity purified polyclonal antibody (Catalog # A02139-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV6 at approximately 83 kDa. The expected band size for TRPV6 is at 87 kDa.
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