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IHC-Fr analysis of rat DRG tissue using GTX54869 TRPV6 antibody. Panel A : TRPV6?channel (red) in DRG neurons. Panel B : Staining with mouse anti-Parvalbumin (green) in the same DRG section. Panel C : Merge of TRPV6?and Parvalbumin demonstrates colocalization (arrows). Dilution : 1:100
IHC-Fr analysis of rat DRG tissue using GTX54869 TRPV6 antibody. Panel A : TRPV6?channel (red) in DRG neurons. Panel B : Staining with mouse anti-Parvalbumin (green) in the same DRG section. Panel C : Merge of TRPV6?and Parvalbumin demonstrates colocalization (arrows). Dilution : 1:100
IHC-Fr analysis of rat DRG tissue using GTX54869 TRPV6 antibody. Panel A : TRPV6?channel (red) in DRG neurons. Panel B : Staining with mouse anti-Parvalbumin (green) in the same DRG section. Panel C : Merge of TRPV6?and Parvalbumin demonstrates colocalization (arrows). Dilution : 1:100

TRPV6 antibody

GTX54869
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRPV6
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRPV6 antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.75 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55503
  • Target name
    TRPV6
  • Target description
    transient receptor potential cation channel subfamily V member 6
  • Target synonyms
    ABP/ZF, CAT1, CATL, ECAC2, HRPTTN, HSA277909, LP6728, ZFAB, transient receptor potential cation channel subfamily V member 6, Alu-binding protein with zinc finger domain, calcium transport protein 1, calcium transporter-like protein, epithelial apical membrane calcium transporter/channel CaT1, epithelial calcium channel 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1D0
  • Protein Name
    Transient receptor potential cation channel subfamily V member 6
  • Scientific Description
    This gene encodes a member of a family of multipass membrane proteins that functions as calcium channels. The encoded protein contains N-terminal ankyrin repeats, which are required for channel assembly and regulation. Translation initiation for this protein occurs at a non-AUG start codon that is decoded as methionine. This gene is situated next to a closely related gene for transient receptor potential cation channel subfamily V member 5 (TRPV5). This locus has experienced positive selection in non-African populations, resulting in several non-synonymous codon differences among individuals of different genetic backgrounds. [provided by RefSeq, Feb 2015]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • TRPV2 channels mediate insulin secretion induced by cell swelling in mouse pancreatic beta-cells. Sawatani T et al., 2019 Mar 1, Am J Physiol Cell Physiol
    Read this paper

Datasheet

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Figure 1. Western blot analysis of TRPV6 using anti-TRPV6 antibody (A02139-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HGC-27 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV6 antigen affinity purified polyclonal antibody (Catalog # A02139-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV6 at approximately 83 kDa. The expected band size for TRPV6 is at 87 kDa.
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