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WB analysis of Molt-4 cell lysate using GTX60167 TRAP antibody. Dilution : 1:1000
WB analysis of Molt-4 cell lysate using GTX60167 TRAP antibody. Dilution : 1:1000
WB analysis of Molt-4 cell lysate using GTX60167 TRAP antibody. Dilution : 1:1000

TRAP antibody

GTX60167
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetACP5
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRAP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:300-1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID54
  • Target name
    ACP5
  • Target description
    acid phosphatase 5, tartrate resistant
  • Target synonyms
    HPAP; human purple acid phosphatase; tartrate-resistant acid ATPase; tartrate-resistant acid phosphatase 5a; tartrate-resistant acid phosphatase 5b; tartrate-resistant acid phosphatase type 5; TRACP5a; TRACP5b; TRAP; TrATPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13686
  • Protein Name
    Tartrate-resistant acid phosphatase type 5
  • Scientific Description
    This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Maternal High-Fat Diet Promotes Abdominal Aortic Aneurysm Expansion in Adult Offspring by Epigenetic Regulation of IRF8-Mediated Osteoclast-like Macrophage Differentiation. Saburi M et al., 2021 Aug 27, Cells
    Read more

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Figure 1. Western blot analysis of Acid Phosphatase/ACP5 using anti-Acid Phosphatase/ACP5 antibody (A03277-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: rat lung tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Acid Phosphatase/ACP5 antigen affinity purified polyclonal antibody (Catalog # A03277-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Acid Phosphatase/ACP5 at approximately 39 kDa. The expected band size for Acid Phosphatase/ACP5 is at 37 kDa.
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