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ProductsBack/TREM1 antibody [L5-B8.2A12.3A12] (Low endotoxin, azide free)

TREM1 antibody [L5-B8.2A12.3A12] (Low endotoxin, azide free)

GTX38898
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetTrem1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TREM1 antibody [L5-B8.2A12.3A12] (Low endotoxin, azide free)
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: . FACS: 1/25-1/100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    L5-B8.2A12.3A12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID58217
  • Target name
    Trem1
  • Target description
    triggering receptor expressed on myeloid cells 1
  • Target synonyms
    triggering receptor expressed on myeloid cells 1, triggering receptor expressed in monocytes 1
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDQ9JKE2
  • Protein Name
    Triggering receptor expressed on myeloid cells 1
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (A02135-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody (Catalog # A02135-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD.
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