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ProductsBack/TRIB2 Mouse Monoclonal Antibody [Clone ID: OTI8D11]
Figure A, Western blot analysis of overexpressed lysates(5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human ROR1 plasmid (RC201210, hTRIB2),mouse TRIB2 plasmid (MR205150, mTRIB2),rat TRIB2 plasmid (RR209102, rTRIB2), using anti-TRIB2 antibody TA500510 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:20000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates(5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human ROR1 plasmid (RC201210, hTRIB2),mouse TRIB2 plasmid (MR205150, mTRIB2),rat TRIB2 plasmid (RR209102, rTRIB2), using anti-TRIB2 antibody TA500510 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:20000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates(5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human ROR1 plasmid (RC201210, hTRIB2),mouse TRIB2 plasmid (MR205150, mTRIB2),rat TRIB2 plasmid (RR209102, rTRIB2), using anti-TRIB2 antibody TA500510 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:20000@1mg/ml).

TRIB2 Mouse Monoclonal Antibody [Clone ID: OTI8D11]

CF500510
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTRIB2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    TRIB2 Mouse Monoclonal Antibody [Clone ID: OTI8D11]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI8D11
  • Gene ID28951
  • Target name
    TRIB2
  • Target description
    tribbles pseudokinase 2
  • Target synonyms
    C5FW, GS3955, TRB2, tribbles homolog 2
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ92519
  • Protein Name
    Tribbles homolog 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) TRIB2 mouse monoclonal antibody, clone OTI8D11 (formerly 8D11)
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of TRIB2 using anti-TRIB2 antibody (PB9900). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIB2 antigen affinity purified polyclonal antibody (Catalog # PB9900) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIB2 at approximately 39 kDa. The expected band size for TRIB2 is at 39 kDa.
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