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TRIM21 Antibody (YA658, Mouse)

HY-P80921
MedChem Express
TargetTRIM21
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TRIM21 Antibody (YA658, Mouse)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6737
  • Target name
    TRIM21
  • Target description
    tripartite motif containing 21
  • Target synonyms
    52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; RING-type E3 ubiquitin transferase TRIM21; RNF81; ro(SS-A); Ro/SSA; Ro/SSA 52kDa; RO52; Sicca syndrome antigen A; sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SSA; SS-A; SSA1; tripartite motif-containing protein 21
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    TRIM21 Antibody (YA658) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIM21.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIM21/SS-A using anti-TRIM21/SS-A antibody (A02079-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM21/SS-A antigen affinity purified polyclonal antibody (Catalog # A02079-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM21/SS-A at approximately 52KD. The expected band size for TRIM21/SS-A is at 52KD.
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