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ProductsBack/TRIM21 / RO52 Antibody

TRIM21 / RO52 Antibody

LS-C405357
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTRIM21
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRIM21 / RO52 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6737
  • Target name
    TRIM21
  • Target description
    tripartite motif containing 21
  • Target synonyms
    RNF81, RO52, Ro/SSA, SSA, SSA1, E3 ubiquitin-protein ligase TRIM21, 52 kDa Ro protein, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, RING finger protein 81, RING-type E3 ubiquitin transferase TRIM21, Ro/SSA 52kDa, SS-A, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro), ro(SS-A), sjoegren syndrome type A antigen, tripartite motif-containing protein 21
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRIM21/SS-A using anti-TRIM21/SS-A antibody (A02079-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM21/SS-A antigen affinity purified polyclonal antibody (Catalog # A02079-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM21/SS-A at approximately 52KD. The expected band size for TRIM21/SS-A is at 52KD.
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