![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100057/GTX100057_44245_20210326_WB_w_23053123_994.webp)
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TRIM23 antibody [N1], N-term
GTX100057
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTRIM23
Overview
- SupplierGeneTex
- Product NameTRIM23 antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.67 mg/ml
- ConjugateUnconjugated
- Gene ID373
- Target nameTRIM23
- Target descriptiontripartite motif containing 23
- Target synonymsARD1, ARFD1, RNF46, E3 ubiquitin-protein ligase TRIM23, ADP-ribosylation factor domain protein 1, 64kDa, ADP-ribosylation factor domain-containing protein 1, ARF domain protein 1, GTP-binding protein ARD-1, RING finger protein 46, RING-type E3 ubiquitin transferase TRIM23, tripartite motif protein TRIM23, tripartite motif-containing protein 23
- HostRabbit
- IsotypeIgG
- Protein IDP36406
- Protein NameE3 ubiquitin-protein ligase TRIM23
- Scientific DescriptionThe protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161

![TRIM23 antibody [N1], N-term detects TRIM23 protein at cytoplasm by immunofluorescent analysis. Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 protein stained by TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining. TRIM23 antibody [N1], N-term detects TRIM23 protein at cytoplasm by immunofluorescent analysis. Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 protein stained by TRIM23 antibody [N1], N-term (GTX100057) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100057/GTX100057_39224_20150410_IFA_w_23053123_829.webp)







