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TRIM24 Antibody (YA2273, Rabbit)

HY-P82528
MedChem Express
TargetTRIM24
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TRIM24 Antibody (YA2273, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8805
  • Target name
    TRIM24
  • Target description
    tripartite motif containing 24
  • Target synonyms
    PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1, transcription intermediary factor 1-alpha, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1-alpha, transcriptional intermediary factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    TRIM24 Antibody (YA2273) is a non-conjugated IgG antibody, targeting TRIM24, with a predicted molecular weight of 117 kDa (observed band size: 117 kDa). TRIM24 Antibody (YA2273) can be used for WB experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIM24 using anti-TRIM24 antibody (A03258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM24 antigen affinity purified polyclonal antibody (Catalog # A03258-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM24 at approximately 130KD. The expected band size for TRIM24 is at 117KD.
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