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TRIM5 Antibody

CAB1872
Assay Genie
TargetTRIM5
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    TRIM5 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID85363
  • Target name
    TRIM5
  • Target description
    tripartite motif containing 5
  • Target synonyms
    RNF88, TRIM5alpha, tripartite motif-containing protein 5, RING-type E3 ubiquitin transferase TRIM5, ring finger protein 88, tripartite motif protein TRIM5, tripartite motif-containing protein 5 alpha
  • Host
    Rabbit
  • Protein IDQ9C035
  • Protein Name
    Tripartite motif-containing protein 5
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, Mouse heart tissue All lanes: TRIM5 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 47, 41, 38, 32, 30 kDa Observed band size: 70 kDa
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Figure 1. Western blot analysis of TRIM5 using anti-TRIM5 antibody (A01359-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM5 antigen affinity purified polyclonal antibody (Catalog # A01359-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM5 at approximately 56KD. The expected band size for TRIM5 is at 56KD.
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