Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Triosephosphate isomerase antibody [C2C3], C-term detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase stained by Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104618/GTX104618_43117_20180307_ICC_IF_w_23060120_945.webp "Triosephosphate isomerase antibody [C2C3], C-term detects Triosephosphate isomerase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase stained by Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10μm.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104618/GTX104618_41752_20161020_WB_shRNA_watermark_w_23060120_117.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term (GTX104618) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Triosephosphate isomerase antibody [C2C3], C-term
GTX104618
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTPI1
Overview
- SupplierGeneTex
- Product NameTriosephosphate isomerase antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.27 mg/ml
- ConjugateUnconjugated
- Gene ID7167
- Target nameTPI1
- Target descriptiontriosephosphate isomerase 1
- Target synonymsHEL-S-49, TIM, TPI, TPID, triosephosphate isomerase, epididymis secretory protein Li 49, methylglyoxal synthase, triose-phosphate isomerase
- HostRabbit
- IsotypeIgG
- Protein IDP60174
- Protein NameTriosephosphate isomerase
- Scientific DescriptionThis gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chen SM, Lin CE, Chen HH, et al. Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass spectrometry. PLoS One. 2020,15(1):e0227838. doi: 10.1371/journal.pone.0227838Read this paper
- Ludvigsen M, Bjerregård Pedersen M, Lystlund Lauridsen K, et al. Proteomic profiling identifies outcome-predictive markers in patients with peripheral T-cell lymphoma, not otherwise specified. Blood Adv. 2018,2(19):2533-2542. doi: 10.1182/bloodadvances.2018019893Read this paper
- Chen JY, Chou HC, Chen YH, et al. High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease. J Nutr Biochem. 2013,24(11):1889-910. doi: 10.1016/j.jnutbio.2013.05.006Read this paper
- Linge A, Kennedy S, O'Flynn D, et al. Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not. Invest Ophthalmol Vis Sci. 2012,53(8):4634-43. doi: 10.1167/iovs.11-9019Read this paper
- Hsiao CY, Hung CY, Tsai TH, et al. A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach. Evid Based Complement Alternat Med. 2012,2012:467531. doi: 10.1155/2012/467531Read this paper
Datasheet
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