More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/TRIT1 antibody [N1C1]

TRIT1 antibody [N1C1]

GTX120508
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTRIT1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    TRIT1 antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID54802
  • Target name
    TRIT1
  • Target description
    tRNA isopentenyltransferase 1
  • Target synonyms
    COXPD35, GRO1, IPPT, IPT, IPTase, MOD5, hGRO1, tRNA dimethylallyltransferase, IPP transferase, isopentenyl-diphosphate:tRNA isopentenyltransferase, tRNA dimethylallyltransferase, mitochondrial, tRNA isopentenylpyrophosphate transferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3H1
  • Protein Name
    tRNA dimethylallyltransferase
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yarham JW, Lamichhane TN, Pyle A, et al. Defective i6A37 modification of mitochondrial and cytosolic tRNAs results from pathogenic mutations in TRIT1 and its substrate tRNA. PLoS Genet. 2014,10(6):e1004424. doi: 10.1371/journal.pgen.1004424
    Read this paper
  • Lamichhane TN, Mattijssen S, Maraia RJ. Human cells have a limited set of tRNA anticodon loop substrates of the tRNA isopentenyltransferase TRIT1 tumor suppressor. Mol Cell Biol. 2013,33(24):4900-8. doi: 10.1128/MCB.01041-13
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western blot All lanes: TRIT1 antibody at 3microg/ml Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 38, 50, 44, 19 kDa Observed band size: 53 kDa
Product group Antibodies
Cusabio
TRIT1 AntibodyCSB-PA867142LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTRIT1
  • SizePrice
Figure 1. Western blot analysis of IPT/TRIT1 using anti-IPT/TRIT1 antibody (A08098). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPT/TRIT1 antigen affinity purified polyclonal antibody (Catalog # A08098) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IPT/TRIT1 at approximately 53 kDa. The expected band size for IPT/TRIT1 is at 53 kDa.
Product group Antibodies
Boster Bio
Anti-IPT/TRIT1 Antibody Picoband(r)A08098-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetTRIT1
  • SizePrice
Product group Antibodies
Biomatik
TRIT1 Polyclonal AntibodyCAC14410
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetTRIT1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRIT1 Polyclonal Antibody, Unconjugated (bs-17134R) at 1:400 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss
TRIT1 Polyclonal AntibodyBS-17134R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetTRIT1
  • SizePrice
Product group Antibodies
Abbexa
TRIT1 Antibody (HRP)ABX310958
ApplicationsELISA
  • SizePrice
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-TRIT1 AntibodyHPA024174
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTRIT1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TRIT1 AntibodyLS-C748159
ApplicationsWestern Blot
TargetTRIT1
  • SizePrice