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TRIT1 Polyclonal Antibody

CAC14410
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTRIT1
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Overview

  • Supplier
    Biomatik
  • Product Name
    TRIT1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54802
  • Target name
    TRIT1
  • Target description
    tRNA isopentenyltransferase 1
  • Target synonyms
    COXPD35, GRO1, IPPT, IPT, IPTase, MOD5, hGRO1, tRNA dimethylallyltransferase, IPP transferase, isopentenyl-diphosphate:tRNA isopentenyltransferase, tRNA dimethylallyltransferase, mitochondrial, tRNA isopentenylpyrophosphate transferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3H1
  • Protein Name
    tRNA dimethylallyltransferase
  • Scientific Description
    The TRIT1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: TRIT1 antibody at 3microg/ml Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 38, 50, 44, 19 kDa Observed band size: 53 kDa
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Figure 1. Western blot analysis of IPT/TRIT1 using anti-IPT/TRIT1 antibody (A08098). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPT/TRIT1 antigen affinity purified polyclonal antibody (Catalog # A08098) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IPT/TRIT1 at approximately 53 kDa. The expected band size for IPT/TRIT1 is at 53 kDa.
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Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRIT1 Polyclonal Antibody, Unconjugated (bs-17134R) at 1:400 overnight at 4°C, DAB staining.
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