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ProductsBack/Trk A Antibody (OAAF05996)

Trk A Antibody (OAAF05996)

OAAF05996
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNTRK1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    Trk A Antibody (OAAF05996)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Gene ID4914
  • Target name
    NTRK1
  • Target description
    neurotrophic receptor tyrosine kinase 1
  • Target synonyms
    MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA, high affinity nerve growth factor receptor, Oncogene TRK, TRK1-transforming tyrosine kinase protein, gp140trk, neurotrophic tyrosine kinase, receptor, type 1, tropomyosin receptor kinase A, tropomyosin-related kinase A, tyrosine kinase receptor A
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of TrkA using anti-TrkA antibody (A00706). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkA antigen affinity purified polyclonal antibody (Catalog # A00706) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkA at approximately 150KD. The expected band size for TrkA is at 87KD.
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