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Trk-A Antibody (YA028, Rabbit)

HY-P80358
MedChem Express
TargetNTRK1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Trk-A Antibody (YA028, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4914
  • Target name
    NTRK1
  • Target description
    neurotrophic receptor tyrosine kinase 1
  • Target synonyms
    MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA, high affinity nerve growth factor receptor, Oncogene TRK, TRK1-transforming tyrosine kinase protein, gp140trk, neurotrophic tyrosine kinase, receptor, type 1, tropomyosin receptor kinase A, tropomyosin-related kinase A, tyrosine kinase receptor A
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Trk-A Antibody (YA028) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Trk-A.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TrkA using anti-TrkA antibody (A00706). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkA antigen affinity purified polyclonal antibody (Catalog # A00706) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkA at approximately 150KD. The expected band size for TrkA is at 87KD.
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