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TRMT61B Antibody

LS-C409606
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTRMT61B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRMT61B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 52kDa, while the observed MW by Western blot was 52kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 52kDa, while the observed MW by Western blot was 52kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55006
  • Target name
    TRMT61B
  • Target description
    tRNA methyltransferase 61B
  • Target synonyms
    tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial, mRNA methyladenosine-N(1)-methyltransferase, potential tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61B, potential tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61B, tRNA methyltransferase 61 homolog B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRMT61B using anti-TRMT61B antibody (A12682-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT61B antigen affinity purified polyclonal antibody (Catalog # A12682-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT61B at approximately 57 kDa. The expected band size for TRMT61B is at 53 kDa.
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