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A549 cell lysates probed with Rabbit Anti-TROP2 Polyclonal Antibody, Unconjugated (bs-6198R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
A549 cell lysates probed with Rabbit Anti-TROP2 Polyclonal Antibody, Unconjugated (bs-6198R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
A549 cell lysates probed with Rabbit Anti-TROP2 Polyclonal Antibody, Unconjugated (bs-6198R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

TROP2 Polyclonal Antibody

BS-6198R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTACSTD2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    TROP2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4070
  • Target name
    TACSTD2
  • Target description
    tumor associated calcium signal transducer 2
  • Target synonyms
    40kD glycoprotein, identified by monoclonal antibody GA733; cell surface glycoprotein TROP2; cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA7331; GA733-1; gastrointestinal tumor-associated antigen GA7331; GP50; M1S1; membrane component, chromosome 1, surface marker 1; pancreatic carcinoma marker protein GA7331; pancreatic carcinoma marker protein GA733-1; TROP2; trophoblast cell surface antigen 2; truncated TACSTD2; tumor-associated calcium signal transducer 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09758
  • Protein Name
    Tumor-associated calcium signal transducer 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TROP2/TACSTD2 using anti-TROP2/TACSTD2 antibody (A03569-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: rat pancreas tissue lysates, Lane 3: mouse pancreas tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TROP2/TACSTD2 antigen affinity purified polyclonal antibody (Catalog # A03569-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TROP2/TACSTD2 at approximately 45-65 kDa. The expected band size for TROP2/TACSTD2 is at 36 kDa.
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