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WB analysis of various sample lysates using GTX32927 Troponin I fast skeletal muscle antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32927 Troponin I fast skeletal muscle antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32927 Troponin I fast skeletal muscle antibody. Dilution : 1:1000 Loading : 25microg per lane

Troponin I fast skeletal muscle antibody

GTX32927
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetTNNI2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Troponin I fast skeletal muscle antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7136
  • Target name
    TNNI2
  • Target description
    troponin I2, fast skeletal type
  • Target synonyms
    AMCD2B; DA2B; DA2B1; FSSV; fsTnI; troponin I fast twitch 2; troponin I type 2 (skeletal, fast); troponin I, fast skeletal muscle; troponin I, skeletal, fast
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48788
  • Protein Name
    Troponin I, fast skeletal muscle
  • Scientific Description
    This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNNI2 using anti-TNNI2 antibody (A07355-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNI2 antigen affinity purified polyclonal antibody (Catalog # A07355-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNI2 at approximately 24KD. The expected band size for TNNI2 is at 21KD.
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