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Troponin I1/TnI1 Rabbit mAb

A9664
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetTNNI1
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Overview

  • Supplier
    ABclonal
  • Product Name
    Troponin I1/TnI1 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID7135
  • Target name
    TNNI1
  • Target description
    troponin I1, slow skeletal type
  • Target synonyms
    SSTNI, TNN1, troponin I, slow skeletal muscle, troponin I type 1 (skeletal, slow)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19237
  • Protein Name
    Troponin I, slow skeletal muscle
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNNI1 using anti-TNNI1 antibody (A07080-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNI1 antigen affinity purified polyclonal antibody (Catalog # A07080-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNI1 at approximately 22 kDa. The expected band size for TNNI1 is at 22 kDa.
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Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE GTX114848 diluted at 1:1000
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