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IHC-Fr analysis of rat DRG tissue using GTX54765 TRPA1 antibody. Panel A : TRPA1 (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). Panel B : Nuclear staining using DAPI as the counterstain. Panel C : Merged image of A and B. Dilution : 1:200
IHC-Fr analysis of rat DRG tissue using GTX54765 TRPA1 antibody. Panel A : TRPA1 (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). Panel B : Nuclear staining using DAPI as the counterstain. Panel C : Merged image of A and B. Dilution : 1:200
IHC-Fr analysis of rat DRG tissue using GTX54765 TRPA1 antibody. Panel A : TRPA1 (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). Panel B : Nuclear staining using DAPI as the counterstain. Panel C : Merged image of A and B. Dilution : 1:200

TRPA1 antibody

GTX54765
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
TargetTRPA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRPA1 antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8989
  • Target name
    TRPA1
  • Target description
    transient receptor potential cation channel subfamily A member 1
  • Target synonyms
    ANKTM1, FEPS, FEPS1, p120, transient receptor potential cation channel subfamily A member 1, ankyrin-like with transmembrane domains 1, transformation-sensitive protein p120, wasabi receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75762
  • Protein Name
    Transient receptor potential cation channel subfamily A member 1
  • Scientific Description
    The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hao M, Song J, Zhai X, et al. Improvement of loperamide-hydrochloride-induced intestinal motility disturbance by Platycodon grandiflorum polysaccharides through effects on gut microbes and colonic serotonin. Front Cell Infect Microbiol. 2023,13:1105272. doi: 10.3389/fcimb.2023.1105272
    Read this paper
  • Liu X, Gong R, Peng L, et al. Toll-like receptor 4 signaling pathway in sensory neurons mediates remifentanil-induced postoperative hyperalgesia via transient receptor potential ankyrin 1. Mol Pain. 2023,19:17448069231158290. doi: 10.1177/17448069231158290
    Read this paper
  • Jiang YH, Jhang JF, Birder LA, et al. Sensory Receptor, Inflammatory, and Apoptotic Protein Expression in the Bladder Urothelium of Patients with Different Subtypes of Interstitial Cystitis/Bladder Pain Syndrome. Int J Mol Sci. 2023,24(1). doi: 10.3390/ijms24010820
    Read this paper
  • Kim SY, Yoon TH, Na J, et al. Mesenchymal Stem Cells and Extracellular Vesicles Derived from Canine Adipose Tissue Ameliorates Inflammation, Skin Barrier Function and Pruritus by Reducing JAK/STAT Signaling in Atopic Dermatitis. Int J Mol Sci. 2022,23(9). doi: 10.3390/ijms23094868
    Read this paper
  • Umehara Y, Toyama S, Tominaga M, et al. Robust induction of neural crest cells to derive peripheral sensory neurons from human induced pluripotent stem cells. Sci Rep. 2020,10(1):4360. doi: 10.1038/s41598-020-60036-z
    Read this paper
  • Chen X, Luo Q, Yan X, et al. Vagal Transient Receptor Potential Ankyrin 1 Mediates Stress-exacerbated Visceral Mechanonociception After Antral Cold Exposure. J Neurogastroenterol Motil. 2019,25(3):442-460. doi: 10.5056/jnm19014
    Read this paper

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Figure 1. Western blot analysis of TRPA1/TSA using anti-TRPA1/TSA antibody (A00453-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPA1/TSA antigen affinity purified polyclonal antibody (Catalog # A00453-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPA1/TSA at approximately 120KD. The expected band size for TRPA1/TSA is at 120KD.
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