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The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA912872(TRPA1 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA912872(TRPA1 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA912872(TRPA1 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)

TRPA1 Antibody

CSB-PA912872
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRPA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRPA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8989
  • Target name
    TRPA1
  • Target description
    transient receptor potential cation channel subfamily A member 1
  • Target synonyms
    ANKTM1; ankyrin-like with transmembrane domains 1; FEPS; FEPS1; transformation-sensitive protein p120; transient receptor potential cation channel subfamily A member 1; wasabi receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75762
  • Protein Name
    Transient receptor potential cation channel subfamily A member 1
  • Scientific Description
    The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRPA1/TSA using anti-TRPA1/TSA antibody (A00453-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPA1/TSA antigen affinity purified polyclonal antibody (Catalog # A00453-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPA1/TSA at approximately 120KD. The expected band size for TRPA1/TSA is at 120KD.
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IHC-Fr analysis of rat DRG tissue using GTX54765 TRPA1 antibody. Panel A : TRPA1 (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). Panel B : Nuclear staining using DAPI as the counterstain. Panel C : Merged image of A and B. Dilution : 1:200
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