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Anti-TRPA1 Antibody

Anti-TRPA1 Antibody

A46164
Antibodies.com
ApplicationsImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-TRPA1 Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to TRPA1
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA912872(TRPA1 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of TRPA1/TSA using anti-TRPA1/TSA antibody (A00453-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPA1/TSA antigen affinity purified polyclonal antibody (Catalog # A00453-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPA1/TSA at approximately 120KD. The expected band size for TRPA1/TSA is at 120KD.
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IHC-Fr analysis of rat DRG tissue using GTX54765 TRPA1 antibody. Panel A : TRPA1 (green) is detected in both cells (horizontal arrow) and processes (vertical arrow). Panel B : Nuclear staining using DAPI as the counterstain. Panel C : Merged image of A and B. Dilution : 1:200
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