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TRPC3 Polyclonal Antibody

RD84678A
Reddot Biotech
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRPC3
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    TRPC3 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7222
  • Target name
    TRPC3
  • Target description
    transient receptor potential cation channel subfamily C member 3
  • Target synonyms
    SCA41, TRP3, short transient receptor potential channel 3, hTrp-3, transient receptor protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPC3 using anti-TRPC3 antibody (A01472-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC3 antigen affinity purified polyclonal antibody (Catalog # A01472-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC3 at approximately 105KD. The expected band size for TRPC3 is at 105KD.
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