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IHC-Fr analysis of rat cortex tissue using GTX54861 TRPC5 antibody. Panel A : TRPC5 (green) appears in pyramidal shaped neuronal soma (vertical arrows) and apical dendrites (horizontal arrows). Panel B : Nuclear staining using DAPI (blue). Panel C : Merged image of A and B.
IHC-Fr analysis of rat cortex tissue using GTX54861 TRPC5 antibody. Panel A : TRPC5 (green) appears in pyramidal shaped neuronal soma (vertical arrows) and apical dendrites (horizontal arrows). Panel B : Nuclear staining using DAPI (blue). Panel C : Merged image of A and B.
IHC-Fr analysis of rat cortex tissue using GTX54861 TRPC5 antibody. Panel A : TRPC5 (green) appears in pyramidal shaped neuronal soma (vertical arrows) and apical dendrites (horizontal arrows). Panel B : Nuclear staining using DAPI (blue). Panel C : Merged image of A and B.

TRPC5 antibody

GTX54861
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRPC5
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRPC5 antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.85 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7224
  • Target name
    TRPC5
  • Target description
    transient receptor potential cation channel subfamily C member 5
  • Target synonyms
    PPP1R159, TRP5, short transient receptor potential channel 5, TRP-5, hTRP5, protein phosphatase 1, regulatory subunit 159, transient receptor potential channel 5, transient receptor protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UL62
  • Protein Name
    Short transient receptor potential channel 5
  • Scientific Description
    This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). [provided by RefSeq, May 2010]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wei L, Mao J, Lu J, et al. Rosiglitazone Inhibits Angiotensin II-Induced Proliferation of Glomerular Mesangial Cells via the Gαq/Plcβ4/TRPC Signaling Pathway. Cell Physiol Biochem. 2017,44(6):2228-2242. doi: 10.1159/000486056
    Read this paper

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Figure 1. Western blot analysis of TRPC5 using anti-TRPC5 antibody (A02701). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC5 antigen affinity purified polyclonal antibody (Catalog # A02701) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC5 at approximately 111KD. The expected band size for TRPC5 is at 111KD.
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