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Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TRPC6 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TRPC6 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TRPC6 Polyclonal Antibody at dilution 1:50

TRPC6 Polyclonal Antibody

E-AB-16106
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TRPC6 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2).
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPC6 using anti-TRPC6 antibody (A00625). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysate,Lane 2: mouse lung tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody (Catalog # A00625) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106KD. The expected band size for TRPC6 is at 106KD.
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