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Mouse probed with Rabbit Anti-TRPM2 Polyclonal Antibody, Unconjugated (bs-2888R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).
Mouse probed with Rabbit Anti-TRPM2 Polyclonal Antibody, Unconjugated (bs-2888R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).
Mouse probed with Rabbit Anti-TRPM2 Polyclonal Antibody, Unconjugated (bs-2888R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).

TRPM2 Polyclonal Antibody

BS-2888R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTRPM2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    TRPM2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7226
  • Target name
    TRPM2
  • Target description
    transient receptor potential cation channel subfamily M member 2
  • Target synonyms
    EREG1; estrogen-responsive element-associated gene 1 protein; KNP3; long transient receptor potential channel 2; LTRPC2; LTrpC-2; NUDT9H; NUDT9L1; transient receptor potential cation channel subfamily M member 2; transient receptor potential channel 7; transient receptor potential melastatin 2; TRPC7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94759
  • Protein Name
    Transient receptor potential cation channel subfamily M member 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPM2 using anti-TRPM2 antibody (A01013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM2 antigen affinity purified polyclonal antibody (Catalog # A01013-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM2 at approximately 171 kDa. The expected band size for TRPM2 is at 171 kDa.
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