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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRPM4 Polyclonal Antibody, Unconjugated (bs-9051R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. _x000D_ _x000D_
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRPM4 Polyclonal Antibody, Unconjugated (bs-9051R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. _x000D_ _x000D_
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRPM4 Polyclonal Antibody, Unconjugated (bs-9051R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. _x000D_ _x000D_

TRPM4 Polyclonal Antibody

BS-9051R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRPM4
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Overview

  • Supplier
    Bioss
  • Product Name
    TRPM4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID54795
  • Target name
    TRPM4
  • Target description
    transient receptor potential cation channel subfamily M member 4
  • Target synonyms
    EKVP6, LTrpC4, PFHB1B, TRPM4B, hTRPM4, transient receptor potential cation channel subfamily M member 4, calcium-activated non-selective cation channel 1, long transient receptor potential channel 4, melastatin-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TD43
  • Protein Name
    Transient receptor potential cation channel subfamily M member 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of TRPM4 using anti-TRPM4 antibody (PB9902). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM4 antigen affinity purified polyclonal antibody (Catalog # PB9902) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM4 at approximately 167 kDa. The expected band size for TRPM4 is at 134 kDa.
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