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Paraformaldehyde-fixed, paraffin embedded Human lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody, Unconjugated (bsm-54661R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody, Unconjugated (bsm-54661R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody, Unconjugated (bsm-54661R) at 1:200 for 30 minutes at room temperature, DAB staining.

Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody

BSM-54661R
Bioss
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetWARS1
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Overview

  • Supplier
    Bioss
  • Product Name
    Tryptophanyl tRNA synthetase / WRS Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IHC()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2C9
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7453
  • Target name
    WARS1
  • Target description
    tryptophanyl-tRNA synthetase 1
  • Target synonyms
    GAMMA-2, HMN9, HMND9, IFI53, IFP53, NEDMSBA, WARS, tryptophan--tRNA ligase, cytoplasmic, epididymis secretory sperm binding protein, hWRS, interferon-induced protein 53, trpRS, tryptophan tRNA ligase 1, cytoplasmic
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23381
  • Protein Name
    Tryptophan--tRNA ligase, cytoplasmic
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of WRS/WARS1 using anti-WRS/WARS1 antibody (A02444-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRS/WARS1 antigen affinity purified polyclonal antibody (Catalog # A02444-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WRS/WARS1 at approximately 55 kDa. The expected band size for WRS/WARS1 is at 53 kDa.
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