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Western Blot analysis of 293 cells using Tuberin Polyclonal Antibody
Western Blot analysis of 293 cells using Tuberin Polyclonal Antibody
Western Blot analysis of 293 cells using Tuberin Polyclonal Antibody

TSC2 Antibody

CSB-PA004343
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTSC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TSC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7249
  • Target name
    TSC2
  • Target description
    TSC complex subunit 2
  • Target synonyms
    LAM, PPP1R160, TSC4, tuberin, protein phosphatase 1, regulatory subunit 160, tuberous sclerosis 2 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49815
  • Protein Name
    Tuberin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tuberin Polyclonal Antibody, Unconjugated (bs-3586R) at 1:200 overnight at 4°C, DAB staining.
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Figure 1. Western blot analysis of Tuberin using anti-Tuberin antibody (PB9121). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human Tuberin protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tuberin antigen affinity purified polyclonal antibody (Catalog # PB9121) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tuberin at approximately 41 kDa. The expected band size for Tuberin is at 41 kDa.
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