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Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining

Tsg101 Polyclonal Antibody

BS-1365R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTSG101
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Overview

  • Supplier
    Bioss
  • Product Name
    Tsg101 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7251
  • Target name
    TSG101
  • Target description
    tumor susceptibility 101
  • Target synonyms
    TSG10, VPS23, tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, tumor susceptibility gene 10, tumor susceptibility gene 101, tumor susceptibility protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99816
  • Protein Name
    Tumor susceptibility gene 101 protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sun Z, Wang L, Zhou Y, et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2020,40(5):767-784. doi: 10.1007/s10571-019-00771-8
    Read this paper
  • Chen Y, Li J, Zhou Y, et al. The role of infectious hematopoietic necrosis virus (IHNV) proteins in recruiting the ESCRT pathway through three ways in the host cells of fish during IHNV budding. Fish Shellfish Immunol. 2019,92:833-841. doi: 10.1016/j.fsi.2019.07.011
    Read this paper

Datasheet

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Figure 1. Western blot analysis of TSG101 using anti-TSG101 antibody (A01233-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human Sw620 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human Hek293 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified polyclonal antibody (Catalog # A01233-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44KD. The expected band size for TSG101 is at 44KD.
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