WB analysis of membrane-enriched extracts (30 microg lysate) of A-431 (Lane 1), IMR-32 (Lane 2) and Jurkat (Lane 3) using GTX25492 TSH receptor antibody [49]. Dilution : 1:250
![ICC/IF analysis of A431 cells using GTX25492 TSH receptor antibody [49]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25492/GTX25492_678_ICC-IF_w_23060722_951.webp "ICC/IF analysis of A431 cells using GTX25492 TSH receptor antibody [49]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature")
![ICC/IF analysis of A431 cells using GTX25492 TSH receptor antibody [49]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25492/GTX25492_679_ICC-IF_w_23060722_407.webp "ICC/IF analysis of A431 cells using GTX25492 TSH receptor antibody [49]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
WB analysis of membrane-enriched extracts (30 microg lysate) of A-431 (Lane 1), IMR-32 (Lane 2) and Jurkat (Lane 3) using GTX25492 TSH receptor antibody [49]. Dilution : 1:250
TSH receptor antibody [49]
GTX25492
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Porcine
TargetTSHR
Overview
- SupplierGeneTex
- Product NameTSH receptor antibody [49]
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes an epitope between amino acid residues 277-296 of human TSHR.
- Application Supplier NoteWB: 1:250. ICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID49
- ConjugateUnconjugated
- Gene ID7253
- Target nameTSHR
- Target descriptionthyroid stimulating hormone receptor
- Target synonymsCHNG1; hTSHR-I; LGR3; seven transmembrane helix receptor; thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; TSH receptor
- HostMouse
- IsotypeIgG1
- Protein IDP16473
- Protein NameThyrotropin receptor
- Scientific DescriptionThe protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
- ReactivityHuman, Porcine
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203