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Immunofluorescent analysis of Hela cells using CSB-PA11189A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using CSB-PA11189A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using CSB-PA11189A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

TSHR Antibody

CSB-PA11189A0RB
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetTSHR
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Overview

  • Supplier
    Cusabio
  • Product Name
    TSHR Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7253
  • Target name
    TSHR
  • Target description
    thyroid stimulating hormone receptor
  • Target synonyms
    CHNG1; hTSHR-I; LGR3; seven transmembrane helix receptor; thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; TSH receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16473
  • Protein Name
    Thyrotropin receptor
  • Scientific Description
    Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin (PubMed:11847099, PubMed:12045258). Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin (PubMed:12045258). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed:11847099). Plays a central role in controlling thyroid cell metabolism (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Human Jurkat cell lysates; Lane 2: Human TT cell lysates probed with TSHR (CT) Polyclonal Antibody, Unconjugated (bs-0206R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of TSH Receptor using anti-TSH Receptor antibody (A00576-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody (Catalog # A00576-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.
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