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ProductsBack/TSH Receptor/TSH-R Antibody (YA1252, Rabbit)

TSH Receptor/TSH-R Antibody (YA1252, Rabbit)

HY-P81507
MedChem Express
TargetTSHR
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TSH Receptor/TSH-R Antibody (YA1252, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7253
  • Target name
    TSHR
  • Target description
    thyroid stimulating hormone receptor
  • Target synonyms
    CHNG1, LGR3, hTSHR-I, thyrotropin receptor, TSH receptor, seven transmembrane helix receptor, thyrotropin receptor-I, hTSHR-I
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    TSH Receptor/TSH-R Antibody (YA1252) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1252), targeting TSH Receptor/TSH-R. TSH Receptor/TSH-R Antibody (YA1252) can be used for IHC-P experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TSH Receptor using anti-TSH Receptor antibody (A00576-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSH Receptor antigen affinity purified polyclonal antibody (Catalog # A00576-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSH Receptor at approximately 86KD. The expected band size for TSH Receptor is at 86KD.
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