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Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: TSR2 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: TSR2 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: TSR2 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa

TSR2 Antibody

CSB-PA836166HA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTSR2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TSR2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID90121
  • Target name
    TSR2
  • Target description
    TSR2 ribosome maturation factor
  • Target synonyms
    DBA14, DT1P1A10, WGG1, pre-rRNA-processing protein TSR2 homolog, TSR2, 20S rRNA accumulation, homolog, WGG motif containing 1, escortin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969E8
  • Protein Name
    Pre-rRNA-processing protein TSR2 homolog
  • Scientific Description
    May be involved in 20S pre-rRNA processing.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TSR2 using anti-TSR2 antibody (A12413-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSR2 antigen affinity purified polyclonal antibody (Catalog # A12413-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSR2 at approximately 24-30 kDa. The expected band size for TSR2 is at 21 kDa.
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