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ProductsBack/TST antibody

TST antibody

GTX114858
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTST
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Overview

  • Supplier
    GeneTex
  • Product Name
    TST antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.71 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7263
  • Target name
    TST
  • Target description
    thiosulfate sulfurtransferase
  • Target synonyms
    epididymis secretory sperm binding protein; RDS; thiosulfate sulfurtransferase; thiosulfate sulfurtransferase (rhodanese)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16762
  • Protein Name
    Thiosulfate sulfurtransferase
  • Scientific Description
    The product of this gene is a mitochondrial matrix enzyme that is encoded by the nucleus. It may play roles in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes. The gene product contains two highly conservative domains (rhodanese homology domains), suggesting these domains have a common evolutionary origin. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • The hepatic compensatory response to elevated systemic sulfide promotes diabetes. Carter RN et al., 2021 Nov 9, Cell Rep
    Read more
  • Sulfide catabolism ameliorates hypoxic brain injury. Marutani E et al., 2021 May 25, Nat Commun
    Read more
  • Inositol-Requiring Enzyme 1alpha Promotes Zika Virus Infection through Regulation of Stearoyl Coenzyme A Desaturase 1-Mediated Lipid Metabolism. Huang Y et al., 2020 Nov 9, J Virol
    Read more
  • Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration. Zivanovic J et al., 2019 Dec 3, Cell Metab
    Read more
  • Cystathionine gamma-lyase deficiency protects mice from galactosamine/lipopolysaccharide-induced acute liver failure. Shirozu K et al., 2014 Jan 10, Antioxid Redox Signal
    Read more
  • Inhaled hydrogen sulfide prevents endotoxin-induced systemic inflammation and improves survival by altering sulfide metabolism in mice. Tokuda K et al., 2012 Jul 1, Antioxid Redox Signal
    Read more

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Figure 1. Western blot analysis of TST using anti-TST antibody (A00965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TST antigen affinity purified polyclonal antibody (Catalog # A00965-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TST at approximately 34 kDa. The expected band size for TST is at 33 kDa.
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