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ProductsBack/TUB / Tubby Antibody

TUB / Tubby Antibody

LS-C662171
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTUB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TUB / Tubby Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7275
  • Target name
    TUB
  • Target description
    TUB bipartite transcription factor
  • Target synonyms
    rd5; RDOB; tubby bipartite transcription factor; tubby homolog; tubby homologue; tubby protein homolog
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TUB 1 using anti-TUB 1 antibody (A02917-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO320 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUB 1 antigen affinity purified polyclonal antibody (Catalog # A02917-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUB 1 at approximately 62 kDa.
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