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IHC-P analysis of human brain tissue using GTX87444 TUBGCP3 antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human brain tissue using GTX87444 TUBGCP3 antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human brain tissue using GTX87444 TUBGCP3 antibody. The picture on the right is blocked with the synthesized peptide.

TUBGCP3 antibody

GTX87444
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTUBGCP3
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Overview

  • Supplier
    GeneTex
  • Product Name
    TUBGCP3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10426
  • Target name
    TUBGCP3
  • Target description
    tubulin gamma complex component 3
  • Target synonyms
    104p, ALP6, GCP3, Grip104, SPBC98, Spc98, Spc98p, gamma-tubulin complex component 3, gamma-ring complex protein 104 kDa, spindle pole body protein Spc98 homolog, tubulin gamma complex associated protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96CW5
  • Protein Name
    Gamma-tubulin complex component 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Dráberová E, D'Agostino L, Caracciolo V, et al. Overexpression and Nucleolar Localization of γ-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma. J Neuropathol Exp Neurol. 2015,74(7):723-42. doi: 10.1097/NEN.0000000000000212
    Read this paper

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Figure 1. Western blot analysis of TUBGCP3 using anti-TUBGCP3 antibody (A10799-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBGCP3 antigen affinity purified polyclonal antibody (Catalog # A10799-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBGCP3 at approximately 104 kDa. The expected band size for TUBGCP3 is at 104 kDa.
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