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ProductsBack/TUSC4 antibody, Internal
WB analysis of human muscle lysate using GTX88896 TUSC4 antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human muscle lysate using GTX88896 TUSC4 antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human muscle lysate using GTX88896 TUSC4 antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer

TUSC4 antibody, Internal

GTX88896
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetNPRL2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TUSC4 antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10641
  • Target name
    NPRL2
  • Target description
    NPR2 like, GATOR1 complex subunit
  • Target synonyms
    FFEVF2, NPR2, NPR2L, TUSC4, GATOR1 complex protein NPRL2, 2810446G01Rik, G21 protein, GATOR complex protein NPRL2, NPR2-like protein, gene 21 protein, homologous to yeast nitrogen permease (candidate tumor suppressor), mutant NPRL2, nitrogen permease regulator 2-like protein, tumor suppressor candidate 4
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ8WTW4
  • Protein Name
    GATOR1 complex protein NPRL2
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of NPRL2 using anti-NPRL2 antibody (A06286-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPRL2 antigen affinity purified polyclonal antibody (A06286-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPRL2 at approximately 48 kDa. The expected band size for NPRL2 is at 44 kDa.
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