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ProductsBack/Twist1 antibody [N1C3]

Twist1 antibody [N1C3]

GTX100621
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetTWIST1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Twist1 antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7291
  • Target name
    TWIST1
  • Target description
    twist family bHLH transcription factor 1
  • Target synonyms
    ACS3; B-HLH DNA binding protein; bHLHa38; BPES2; BPES3; class A basic helix-loop-helix protein 38; CRS; CRS1; CSO; H-twist; SCS; SWCOS; TWIST; twist basic helix-loop-helix transcription factor 1; twist homolog 1; TWIST homolog of drosophila; twist-related protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15672
  • Protein Name
    Twist-related protein 1
  • Scientific Description
    Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Eriodictyol inhibits glioblastoma migration and invasion by reversing EMT via downregulation of the P38 MAPK/GSK-3beta/ZEB1 pathway. Lv F et al., 2021 Jun 5, Eur J Pharmacol
    Read more
  • Reverse epithelial-mesenchymal transition contributes to the regain of drug sensitivity in tyrosine kinase inhibitor-resistant non-small cell lung cancer cells. Lee AF et al., 2017, PLoS One
    Read more

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Figure 1. Western blot analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat ovary tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse ovary tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWIST1/2 antigen affinity purified polyclonal antibody (Catalog # A00980-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWIST1/2 at approximately 30 kDa. The expected band size for TWIST1/2 is at 21 kDa.
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