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Formalin-fixed and paraffin embedded human osteosarcoma tissue labeled with Anti-Twist Polyclonal Antibody (bs-2441R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded human osteosarcoma tissue labeled with Anti-Twist Polyclonal Antibody (bs-2441R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded human osteosarcoma tissue labeled with Anti-Twist Polyclonal Antibody (bs-2441R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining

TWIST Polyclonal Antibody

BS-2441R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTWIST1
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Overview

  • Supplier
    Bioss
  • Product Name
    TWIST Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7291
  • Target name
    TWIST1
  • Target description
    twist family bHLH transcription factor 1
  • Target synonyms
    ACS3, BPES2, BPES3, CRS, CRS1, CSO, SCS, SWCOS, TWIST, bHLHa38, twist-related protein 1, B-HLH DNA binding protein, H-twist, TWIST homolog of drosophila, class A basic helix-loop-helix protein 38, twist basic helix-loop-helix transcription factor 1, twist homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15672
  • Protein Name
    Twist-related protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Chang J, Gao F, Chu H, et al. miR-363-3p inhibits migration, invasion, and epithelial-mesenchymal transition by targeting NEDD9 and SOX4 in non-small-cell lung cancer. J Cell Physiol. 2020,235(2):1808-1820. doi: 10.1002/jcp.29099
    Read this paper
  • Ma T, Zhao Z, Wang Z, et al. MiR-940 inhibits migration and invasion of tongue squamous cell carcinoma via regulatingCXCR2/NF-κB system-mediated epithelial-mesenchymal transition. Naunyn Schmiedebergs Arch Pharmacol. 2019,392(11):1359-1369. doi: 10.1007/s00210-019-01671-w
    Read this paper
  • Shen T, Cai LD, Liu YH, et al. Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy. J Hematol Oncol. 2018,11(1):95. doi: 10.1186/s13045-018-0638-9
    Read this paper
  • Wang Q, Li H, Sun Z, et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. Sci Rep. 2016,6:36543. doi: 10.1038/srep36543
    Read this paper

Datasheet

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Figure 1. Western blot analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat ovary tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse ovary tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWIST1/2 antigen affinity purified polyclonal antibody (Catalog # A00980-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWIST1/2 at approximately 30 kDa. The expected band size for TWIST1/2 is at 21 kDa.
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