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Western blot analysis of extracts of various cell lines using Twist Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using Twist Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using Twist Polyclonal Antibody at dilution of 1:1000.

TWIST1 Polyclonal Antibody

E-AB-65429
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TWIST1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat ovary tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse ovary tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWIST1/2 antigen affinity purified polyclonal antibody (Catalog # A00980-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWIST1/2 at approximately 30 kDa. The expected band size for TWIST1/2 is at 21 kDa.
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