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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-TXNIP Polyclonal Antibody, Unconjugated (bs-3897R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-TXNIP Polyclonal Antibody, Unconjugated (bs-3897R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-TXNIP Polyclonal Antibody, Unconjugated (bs-3897R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n

TXNIP Polyclonal Antibody

BS-3897R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTXNIP
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Overview

  • Supplier
    Bioss
  • Product Name
    TXNIP Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID10628
  • Target name
    TXNIP
  • Target description
    thioredoxin interacting protein
  • Target synonyms
    ARRDC6, EST01027, HHCPA78, THIF, VDUP1, thioredoxin-interacting protein, thioredoxin binding protein 2, upregulated by 1,25-dihydroxyvitamin D-3, vitamin D3 up-regulated protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3M7
  • Protein Name
    Thioredoxin-interacting protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Liu Z, Sun M, Wang Y, et al. Silymarin attenuated paraquat-induced cytotoxicity in macrophage by regulating Trx/TXNIP complex, inhibiting NLRP3 inflammasome activation and apoptosis. Toxicol In Vitro. 2018,46:265-272. doi: 10.1016/j.tiv.2017.10.017
    Read this paper

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Figure 1. Western blot analysis of TXNIP using anti-TXNIP antibody (A01409-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse NIH-3T3 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: human K562 whole cell lysates, Lane 9: human Hela whole cell lysates, Lane 10: human HEK293 whole cell lysates, Lane 11: human HL-60 whole cell lysates, Lane 12: human A549 whole cell lysates, Lane 13: human Caco-2 whole cell lysates, Lane 14: human Raji whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNIP antigen affinity purified polyclonal antibody (Catalog # A01409-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNIP at approximately 44KD. The expected band size for TXNIP is at 44KD.
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