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Anti-TXNIP Antibody

144-66751
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetTXNIP
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TXNIP Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID10628
  • Target name
    TXNIP
  • Target description
    thioredoxin interacting protein
  • Target synonyms
    ARRDC6, EST01027, HHCPA78, THIF, VDUP1, thioredoxin-interacting protein, thioredoxin binding protein 2, upregulated by 1,25-dihydroxyvitamin D-3, vitamin D3 up-regulated protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3M7
  • Protein Name
    Thioredoxin-interacting protein
  • Scientific Description
    TXNIP pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TXNIP using anti-TXNIP antibody (A01409-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse NIH-3T3 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: human K562 whole cell lysates, Lane 9: human Hela whole cell lysates, Lane 10: human HEK293 whole cell lysates, Lane 11: human HL-60 whole cell lysates, Lane 12: human A549 whole cell lysates, Lane 13: human Caco-2 whole cell lysates, Lane 14: human Raji whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNIP antigen affinity purified polyclonal antibody (Catalog # A01409-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNIP at approximately 44KD. The expected band size for TXNIP is at 44KD.
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