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WB analysis of various samples using GTX04756 TYRO3 antibody [ARC1841]. Loading : 25 microg Dilution : 1:1000
WB analysis of various samples using GTX04756 TYRO3 antibody [ARC1841]. Loading : 25 microg Dilution : 1:1000
WB analysis of various samples using GTX04756 TYRO3 antibody [ARC1841]. Loading : 25 microg Dilution : 1:1000

TYRO3 antibody [ARC1841]

GTX04756
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetTYRO3
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Overview

  • Supplier
    GeneTex
  • Product Name
    TYRO3 antibody [ARC1841]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ARC1841
  • Conjugate
    Unconjugated
  • Gene ID7301
  • Target name
    TYRO3
  • Target description
    TYRO3 protein tyrosine kinase
  • Target synonyms
    BYK; Dtk; Etk-2; Rek; RSE; Sky; Tif; tyrosine-protein kinase byk; tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; tyrosine-protein kinase RSE; tyrosine-protein kinase SKY; tyrosine-protein kinase TIF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06418
  • Protein Name
    Tyrosine-protein kinase receptor TYRO3
  • Scientific Description
    The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses. [provided by RefSeq, May 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TYRO3 using anti-TYRO3 antibody (M00913-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYRO3 antigen affinity purified monoclonal antibody (Catalog # M00913-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYRO3 at approximately 120 kDa. The expected band size for TYRO3 is at 97 kDa.
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IHC-P analysis of human breast adenocarcinoma tissue using GTX83459 TYRO3 antibody [5B4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
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